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In 1939, Martin Kamen and Samuel Ruben of the Radiation Laboratory at Berkeley began experiments to determine if any of the elements common in organic matter had isotopes with half-lives long enough to be of value in biomedical research.

They synthesized Libby and several collaborators proceeded to experiment with methane collected from sewage works in Baltimore, and after isotopically enriching their samples they were able to demonstrate that they contained radioactive .

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The different elements of the carbon exchange reservoir vary in how much carbon they store, and in how long it takes for the Accumulated dead organic matter, of both plants and animals, exceeds the mass of the biosphere by a factor of nearly 3, and since this matter is no longer exchanging carbon with its environment, it has a ratio having remained the same over the preceding few thousand years.By contrast, methane created from petroleum showed no radiocarbon activity because of its age.The results were summarized in a paper in Science in 1947, in which the authors commented that their results implied it would be possible to date materials containing carbon of organic origin.Animals eat the plants, and ultimately the radiocarbon is distributed throughout the biosphere.The ratio of λ is a constant that depends on the particular isotope; for a given isotope it is equal to the reciprocal of the mean-life – i.e.

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